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Adjacent to the 5′ LTR are sequences necessary for reverse transcription of the genome (the tRNA primer binding site) and for efficient packaging of viral RNA into particles (the Psi site). The U5 region is the sequence between the primer binding site and the R region and contains the polyadenylation sequence. The LTR contains numerous regulatory signals including transcriptional control elements, polyadenylation signals and sequences needed for replication and integration of the viral genome. Where reference is made herein to elements such as cloning sites, promoters, regulatory elements, heterologous nucleic acids, etc., it is to be understood that the sequences of these elements are present in RNA form in the lentiviral particles of the invention and are present in DNA form in the DNA plasmids of the invention. Thus, as used herein, the terms “packaging sequence,” “packaging signal,” “psi” and the symbol “.PSI.,” are used in reference to the non-coding… read more
When parents and educators fail you, and peers lack credibility, where else are you to turn? Ballerini’s widespread appeal of course stems first from her music, which incorporates more pop production than many of her country peers — something especially prevalent on Kelsea. In preferred embodiments, the pharmaceutical T cell compositions comprises potent T cells having one or more of, or all of the following markers: CD62L, CCR7, CD28, CD27, CD122, and CD127. In certain embodiments, the compositions described herein are administered in conjunction with a cytokine. In certain embodiments, compositions comprising T cells contemplated herein may be administered in conjunction with any number of chemotherapeutic agents. A variety of other therapeutic agents may be used in conjunction with the compositions described herein. Generally, compositions comprising the cells activated and expanded as described herein may be utilized in the treatment and prevention of diseases that arise in individuals who are… read more